simple chip plus sonication chromatin ip kit  (Cell Signaling Technology Inc)

Journal: Cell Death Discovery

Article Title: Taurine is a natural suppressor of urea cycle via targeting ASL

doi: 10.1038/s41420-026-02959-6

Figure Lengend Snippet: A Putative FOS:JUN binding consensus sequences in promoter of ASL (bottom) were compared with motif predicted using JASPAR database (top). B , C MHCC97H cells were treated with or without 100 μM taurine for the indicated days. Immunoblotting analysis was performed using the indicated antibodies ( B ). mRNA levels of FOS were analyzed ( C ). D Plasmid containing either WT or mutant promoter sequence of ASL was co-transfected with FOS into 293T cells. Dual luciferase reporter assay was performed. E , F MHCC97H cells were transfected with or without sgRNA targeting FOS (sgFOS) and treated with or without 100 μM taurine for four days. Immunoblotting analysis was performed using the indicated antibodies ( E ). mRNA levels of ASL were analyzed ( F ). G ChIP assay was performed in sgCtrl and sgFOS MHCC97H cells using antibodies against c-JUN. DNA enrichment was examined by quantitative real-time PCR. The y axis shows the value normalized to input. H - J sgCtrl and sgFOS MHCC97H cells were treated with or without 100 μM taurine for four days. Arginine and urea levels ( H ) ammonia levels ( I ) and urea release ( J ) were analyzed. K Cell viability of sgCtrl and sgFOS MHCC97H cells treated with or without 100 μM taurine for indicated days were analyzed. Data are presented as mean ± SD, n = 3 independent repeats. Unpaired, two-tailed t test; * P < 0.05; ** P < 0.01. NS, not significant.

Article Snippet: ChIP assay kit (56383) was purchased from Cell Signaling Technology.

Techniques: Binding Assay, Western Blot, Plasmid Preparation, Mutagenesis, Sequencing, Transfection, Luciferase, Reporter Assay, Real-time Polymerase Chain Reaction, Two Tailed Test